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您現(xiàn)在的位置：首頁(yè) >> 產(chǎn)品中心 >> 生化試劑、抗體、血清 >> 試劑盒 >> 12381QIAGEN EndoFree Plasmid Mega Kit無(wú)內(nèi)毒素質(zhì)粒提取試劑盒

QIAGEN EndoFree Plasmid Mega Kit無(wú)內(nèi)毒素質(zhì)粒提取試劑盒

更新時(shí)間： 2023-07-26
產(chǎn)品型號(hào)： 12381
簡(jiǎn)單描述
QIAGEN EndoFree Plasmid Mega Kit無(wú)內(nèi)毒素質(zhì)粒提取試劑盒
EndoFree Plasmid Kits基于陰離子交換技術(shù)，可快速制備不含內(nèi)毒素的質(zhì)粒DNA。QIAfilter Cartridges過(guò)濾快速澄清裂解液。制備的DNA純度超過(guò)兩次CsCl梯度離心法所得DNA的純度，適合超轉(zhuǎn)染級(jí)純的應(yīng)用。EndoFree Plasmid Buffer Set可用于制備10次m

詳細(xì)介紹

QIAGEN EndoFree Plasmid Mega Kit無(wú)內(nèi)毒素質(zhì)粒提取試劑盒

純化得到至多10 mg的無(wú)內(nèi)毒素高轉(zhuǎn)染級(jí)純質(zhì)粒或柯斯質(zhì)粒DNA

純化得到的DNA內(nèi)毒素水平低于0.1 EU/µg
純化流程快，同時(shí)去除內(nèi)毒素
獲得高產(chǎn)量、高拷貝質(zhì)粒DNA
使用LyseBlue，獲得的裂解和高產(chǎn)量的DNA

QIAGEN EndoFree Plasmid Mega Kit無(wú)內(nèi)毒素質(zhì)粒提取試劑盒

質(zhì)粒純化方法與轉(zhuǎn)染效率。

使用兩種方法獨(dú)立制備pRSVcat，分別使用脂質(zhì)體介導(dǎo)的方法轉(zhuǎn)染到COS-7、HeLa和LMH細(xì)胞中，及使用磷酸鈣轉(zhuǎn)染至Huh7細(xì)胞中。平均轉(zhuǎn)染效率以相對(duì)于QIAGEN Plasmid Kit（100％）制備的DNA的轉(zhuǎn)染效率表示。

質(zhì)粒純化方法與轉(zhuǎn)染效率。

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EndoFree Plasmid Maxi Kit (10)	3890	12362	10
EndoFree Plasmid Mega Kit (5)	4720	12381	5
EndoFree Plasmid Giga Kit (5)	8080	12391	5
QIAGEN Large-Construct Kit (10)	4850	12462	10
HiSpeed Plasmid Midi Kit (25)	3840	12643	25
HiSpeed Plasmid Maxi Kit (10)	3180	12662	10
HiSpeed Plasmid Maxi Kit (25)	7440	12663	25
CompactPrep Plasmid Midi Kit (25)	3010	12843	25
CompactPrep Plasmid Maxi Kit (25)	6120	12863	25
QIAGEN Plasmid Plus Midi Sample Kit (5)	723	12941
QIAGEN Plasmid Plus Midi Kit (25)	3600	12943	25
QIAGEN Plasmid Plus Midi Kit (100)	13240	12945
QIAGEN Plasmid Plus Maxi Sample Kit (5)	1490	12961
QIAGEN Plasmid Plus Maxi Kit (25)	7330	12963	25
QIAGEN Plasmid Plus Maxi Kit (100)	27180	12965
QIAGEN Plasmid Plus Mega Kit (5)	3980	12981	5
QIAGEN Plasmid Plus Giga Kit (5)	6950	12991
QIAGEN Blood&CellCulture DNA MiniKit(25)	3390	13323	25
Blood & Cell Culture DNA Midi Kit (25)	5400	13343	25
QIAGEN Blood&CellCulture DNA MaxiKit(10)	5070	13362	10
QIAGEN Plasmid Plus 96 Miniprep Kit (4)	35860	16181	384
QIArack	1250	19015
Plasmid Buffer Set	1410	19046
EndoFree Plasmid Buffer Set	7430	19048
Buffer P1 (500 ml)	811	19051	500
Buffer P2 (500ml)	811	19052	500
Buffer P3 (500ml)	811	19053	500
Buffer QBT (1000ml)	1410	19054	1000
Buffer QC (1000ml)	1410	19055	1000
Buffer QF (1000ml)	1410	19056	1000
Genomic DNA Buffer Set	2080	19060	75
Buffer QG (250 ml)	666	19063	250
Buffer N3 (500ml)	1060	19064	500
Buffer PE (concentrate, 100ml)	1060	19065	100
Buffer PB (500ml)	1060	19066	500
Buffer AW/AW2 (concentrate, 324 ml)	1220	19072	324
Buffer AVL (155 ml)	1900	19073
Buffer AL	1220	19075	1
Buffer ATL (200ml)	1220	19076	200
Buffer AE (240ml)	1220	19077	1
Buffer AW1 (concentrate, 242 ml)	1220	19081	1
Buffer ASL (4 x 140 ml)	1370	19082
Buffer PM (500ml)	837	19083	500
Buffer EB (250ml)	423	19086	250
Reagent DX	663	19088	1
Buffer RPW (125ml)	598	19090	125
Buffer MTL (54 ml)	466	19112	54
QIAGEN Proteinase K (2ml)	1180	19131	2
QIAGEN Proteinase K (10 ml)	4200	19133	1
QIAGEN Protease (7.5 AU)	996	19155
QIAGEN Protease (30 AU)	3200	19157	30
Collection Tubes (2-ml)	1830	19201
VacConnectors (500)	1820	19407
VacValves (24)	1250	19408
QIAvac 24 Plus	4300	19413
QIAvac Holder	1990	19418
QIAvac Connecting System	13950	19419
QIAvac 96	10200	19504
Collection Microtubes (racked, 10 x 96)	2120	19560
Collection Microtube Caps (120 x 8)	439	19566
Tape Pads (5)	822	19570	5
QIAEX II Gel Extraction Kit (150)	2340	20021	150
QIAEX II Gel Extraction Kit (500)	5350	20051	500
QIAEX II Suspension (1.5ml)	1840	20902	1
Buffer QX1 (500ml)	1110	20912	500
R.E.A.L. Prep 96 Plasmid Kit (24)	54800	26173	2304
QIAprep Spin Miniprep Kit (50)	1070	27104	50
QIAprep Spin Miniprep Kit (250)	4100	27106	250
QIAprep 2.0 Spin Columns (100)	1130	27115	100
QIAprep 96 Turbo Core Kit (24)	64690	27173	2304
QIAprep 96 Turbo Miniprep Kit (4)	13660	27191	384
QIAprep 96 Turbo Miniprep Kit (24)	77320	27193	2304
QIAprep 96 Plus Miniprep Kit (4)	15610	27291	384
DirectPrep 96 Miniprep Kit (4)	7650	27361	384
QuickLyse Miniprep Kit (100)	2420	27405	100
QuickLyse Miniprep Kit (250)	5190	27406	250
MinElute PCR Purification Kit (50)	1330	28004	50
MinElute PCR Purification Kit (250)	6000	28006	250
MinElute 96 UF PCR Purification Kit (4)	3710	28051	384
MinElute 96 UF PCR Purification Kit (24)	14740	28053	2304
QIAquick PCR Purification Kit (50)	1100	28104	50
QIAquick PCR Purification Kit (250)	5150	28106	250
QIAquick 96 PCR Purification Kit (4)	10180	28181	384
QIAquick 96 PCR Purification Kit (24)	40340	28183	2304
MinElute Reaction Cleanup Kit (50)	1600	28204	50
MinElute Reaction Cleanup Kit (250)	7340	28206	250
QIAquick Nucleotide Removal Kit (50)	1410	28304	50
QIAquick Nucleotide Removal Kit (250)	6610	28306	250
MinElute Gel Extraction Kit (50)	1550	28604	50
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Ni-NTA Agarose (25 ml)	3850	30210	25
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Ni-NTA Agarose (500 ml)	56230	30250	500
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Ni-NTA Superflow (25 ml)	4740	30410	25
Ni-NTA Superflow (100 ml)	15920	30430	100
Ni-NTA Superflow (500 ml)	67410	30450	500
Ni-NTA Fast Start Kit (6)	4350	30600	6
Ni-NTA Superflow Columns (12 x 1.5 ml)	5200	30622	12
Ni-NTA Superflow Cartridges (5x1 ml)	2210	30721	5
Ni-NTA Superflow Cartridges (1x5 ml)	1960	30760	1
Ni-NTA Superflow Cartridges (5x5 ml)	7670	30761	5
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QIAamp DNA Blood Midi Kit (20)	2590	51183	20
QIAamp DNA Blood Midi Kit (100)	11220	51185	100
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QIAamp DNA Blood Maxi Kit (50)	10880	51194	50
FlexiGene DNA Kit (250)	3750	51206	250
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QIAamp 96 DNA QIAcube HT Kit (5)	8790	51331	480
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QIAamp DNA Stool Mini Kit (50)	2870	51504	50
QIAamp Fast DNA Stool Mini Kit (50)	3190	51604	50
QIAamp DNA Microbiome Kit (50)	6620	51704	50
QIAamp RNA Blood Mini Kit (50)	4250	52304	50
QIAamp Viral RNA Mini Kit (50)	3010	52904	50
QIAamp Viral RNA Mini Kit (250)	12980	52906	250
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QIAamp UltraSens Virus Kit (50)	6030	53704	50
QIAamp UltraSens Virus Kit (250)	25230	53706	250
QIAamp cador Pathogen Mini Kit (50)	2560	54104	50
QIAamp cador Pathogen Mini Kit (250)	12210	54106	250
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QIAamp DNA Micro Kit (50)	2830	56304	50
QIAamp DNA FFPE Tissue Kit (50)	2590	56404	50
QIAamp DNA Investigator Kit (50)	2990	56504	50
QIAamp MinElute Media Kit (50)	4860	57414	50
QIAamp MinElute Virus Spin Kit (50)	3180	57704	50
QIAamp MinElute Virus Vacuum Kit (50)	4060	57714	50
QIAamp 96 Virus QIAcube HT Kit (5)	16960	57731	480
EpiTect Bisulfite Kit (48)	2800	59104	48
EpiTect 96 Bisulfite Kit (2)	9990	59110	192
EpiTect Plus DNA Bisulfite Kit (48)	2250	59124	48
EpiTect Whole Bisulfitome Kit (25)	4560	59203	25
EpiTect MSP Kit (100)	3600	59305	100
EpiTect MethyLight PCR Kit (200)	10340	59436	200
EpiTect HRM PCR Kit (100)	1440	59445	100
EpiTect MethyLight PCR +ROX Kit (200)	10600	59496	200
EpiTect Control DNA (1000)	2730	59568	1000
EpiTect Control DNA, methylated (100)	2730	59655	100
EpiTect Control DNA, unmethylated (100)	2730	59665	100
EpiTect PCR Control DNA Set (100)	5460	59695	300
EpiTect Fast 96 DNA Bisulfite Kit	10910	59720	192
EpiTect Fast 96 FFPE Bisulfite Kit	19310	59740	192
EpiTect Fast Bisulfite Kit (10)	847	59802	10
EpiTect Fast DNA Bisulfite Kit (50)	2430	59824	50
EpiTect Fast DNA Bisulfite Kit (200)	8290	59826	200
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EpiTect Fast LyseAll Bisulfite Kit (50)	2720	59864	50
EpiTect Fast LyseAll Bisulfite Kit (200)	9270	59866	200
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DNeasy Plant Mini Kit (50)	2750	69104	50
DNeasy Plant Mini Kit (250)	12310	69106	250
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DNeasy Blood & Tissue Kit (250)	8810	69506	250
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TissueLyser LT Adapter, 12-Tube	17250	69980
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